orbital microplate shaker (Fisher Scientific)
86
Structured Review
Fisher Scientific
orbital microplate shaker
Orbital Microplate Shaker, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microplate+shaker/fisher+microplate+scientific+shaker/pm42285030-91-18-21
Average 86 stars, based on 1 article reviews
Orbital Microplate Shaker, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microplate+shaker/fisher+microplate+scientific+shaker/pm42285030-91-18-21
Average 86 stars, based on 1 article reviews
orbital microplate shaker - by Bioz Stars,
2026-10
86/100 stars
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Transferring:Article Title: P450-Catalyzed Atom Transfer Radical Cyclization Article Snippet: 2.Transfer 100 μL sterile 50% glycerol to each well of a separate 96-well microplate for glycerol stock storage.Transfer 100 μL LBamp culture from the starter culture plate to each well of this microplate.Cover the lid and mix it by gentle swirling.Keep the bacterial glycerol stock in the microplate in a −80 °C freezer.Thaw the 1000X IPTG and 1000X ALA stock from the −20 °C freezer.After 2.5 h, put the 96-well plate with expression culture on ice for 20 min.Prepare the IPTG/ALA stock solution by mixing 20 mL HBamp media with 400 μL 1000X IPTG and 400 μL 1000X ALA stock solutions.Transfer 50 μL of this newly prepared, diluted IPTG/ALA solution to each well of the 96-well plate containing expression cultures using an Eppendorf Xplorer 12-channel pipette.Gently shake the plate to ensure good mixing.Re-cover the 96-well plate with the microporous film.Incubate the expression culture at 22 °C, 220 rpm for 22 h in a New Brunswick Innova 44R shaker. .. 3 Set up screening reactions with the whole E. coli cells in 96-well plate: Cool the Eppendorf tabletop centrifuge 5910R to 4 °C (takes 30 min).Put the 96-well plate with HBamp culture on ice.Spin down the cell pellets at 3000 rpm at 4 °C for 3 min and discard the supernatant.Transfer 345 μL M9-N buffer and 40 μL D-Glucose solution (0.5 M in M9-N buffer) to each well and shake the plate in a Article Title: P450-Catalyzed Atom Transfer Radical Cyclization Article Snippet: 2.Transfer 100 μL sterile 50% glycerol to each well of a separate 96-well microplate for glycerol stock storage.Transfer 100 μL LBamp culture from the starter culture plate to each well of this microplate.Cover the lid and mix it by gentle swirling.Keep the bacterial glycerol stock in the microplate in a −80 °C freezer.Thaw the 1000X IPTG and 1000X ALA stock from the −20 °C freezer.After 2.5 h, put the 96-well plate with expression culture on ice for 20 min.Prepare the IPTG/ALA stock solution by mixing 20 mL HBamp media with 400 μL 1000X IPTG and 400 μL 1000X ALA stock solutions.Transfer 50 μL of this newly prepared, diluted IPTG/ALA solution to each well of the 96-well plate containing expression cultures using an Eppendorf Xplorer 12-channel pipette.Gently shake the plate to ensure good mixing.Re-cover the 96-well plate with the microporous film.Incubate the expression culture at 22 °C, 220 rpm for 22 h in a New Brunswick Innova 44R shaker. .. 3 3 Set up screening reactions with the whole E. coli cells in 96-well plate: Cool the Eppendorf tabletop centrifuge 5910R to 4 °C (takes 30 min).Put the 96-well plate with HBamp culture on ice.Spin down the cell pellets at 3000 rpm at 4 °C for 3 min and discard the supernatant.Transfer 345 μL M9-N buffer and 40 μL D-Glucose solution (0.5 M in M9-N buffer) to each well and shake the plate in a Plasmid Purification:Article Title: Directed Evolution and Unusual Protonation Mechanism of Pyridoxal Radical C-C Coupling Enzymes for the Enantiodivergent Photobiocatalytic Synthesis of Noncanonical Amino Acids. Article Snippet: E. coli (E. cloni BL21(DE3)) cells in deep-well 96- well plates were harvested by centrifugation (3,000 rpm, 5 min, 4 C) using an Eppendorf tabletop centrifuge 5910R. .. The cell pellets were resuspended in KPi buffer (200 mM, pH 7.0, 700 μL/well) containing 0.2 mM PLP by gentle shaking (800 rpm, 3–5 min) on a Gentle:Article Title: Directed Evolution and Unusual Protonation Mechanism of Pyridoxal Radical C-C Coupling Enzymes for the Enantiodivergent Photobiocatalytic Synthesis of Noncanonical Amino Acids. Article Snippet: E. coli (E. cloni BL21(DE3)) cells in deep-well 96- well plates were harvested by centrifugation (3,000 rpm, 5 min, 4 C) using an Eppendorf tabletop centrifuge 5910R. .. The cell pellets were resuspended in KPi buffer (200 mM, pH 7.0, 700 μL/well) containing 0.2 mM PLP by gentle shaking (800 rpm, 3–5 min) on a Article Title: Enzymatic Synthesis of Unprotected α,β-Diamino Acids via Direct Asymmetric Mannich Reactions. Article Snippet: Supporting Information Enzymatic synthesis of unprotected a,b-diamino acids via direct asymmetric Mannich reactions Shaonan Liu1, Jinmin Gao1, Yike Zou,2,3 Yang Hai1* Incubation:Article Title: Enzymatic Synthesis of Unprotected α,β-Diamino Acids via Direct Asymmetric Mannich Reactions. Article Snippet: Supporting Information Enzymatic synthesis of unprotected a,b-diamino acids via direct asymmetric Mannich reactions Shaonan Liu1, Jinmin Gao1, Yike Zou,2,3 Yang Hai1* |